Construct: shRNA TRCN0000221130
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004720.4-1658s1c2, TRCN0000011374, NM_004720.4-1658s1c1
- DNA Barcode:
- TCTACCTGAGTCTGATTCTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LPAR2 (9170)
- Gene Description:
- lysophosphatidic acid receptor 2
- Transcript:
- RefSeq NM_004720.4 (NON-CURRENT)
- Match location:
- Position 1658 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000221130)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCTACCTGAGTCTGATTCTTT
- Hairpin Sequence:
-
5'-CCGG-TCTACCTGAGTCTGATTCTTT-CTCGAG-AAAGAATCAGACTCAGGTAGA-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCTACCTGAGTCTGATTCTTTCTCGAGAAAGAATCAGACTCAGGTAGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCTACCTGAGTCTGATTCTTTCTCGAGAAAGAATCAGACTCAGGTAGA-3'
Other clones with same target sequence:
(none)
