Construct: shRNA TRCN0000221157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022726.2-983s1c2, TRCN0000011405, NM_022726.2-983s1c1
DNA Barcode:
GCCTATGCAATCAGCTTCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELOVL4 (6785)
Gene Description:
ELOVL fatty acid elongase 4
Transcript:
RefSeq NM_022726.2 (NON-CURRENT)
Match location:
Position 983 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATGCAATCAGCTTCATA
Hairpin Sequence:
5'-CCGG-GCCTATGCAATCAGCTTCATA-CTCGAG-TATGAAGCTGATTGCATAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATGCAATCAGCTTCATACTCGAGTATGAAGCTGATTGCATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATGCAATCAGCTTCATACTCGAGTATGAAGCTGATTGCATAGGC-3'

Other clones with same target sequence:

(none)