Construct: shRNA TRCN0000221173

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358135.1-132s1c2, TRCN0000024076, XM_358135.1-132s1c1
DNA Barcode:
CTTGAGTGATAAAGAACCTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC385236 (385236)
Gene Description:
similar to Serine/threonine-protein kinase tousled-like 2 (Tousled-like kinase 2)
Transcript:
RefSeq XM_358135.1 (NON-CURRENT)
Match location:
Position 132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221173)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGAGTGATAAAGAACCTCT
Hairpin Sequence:
5'-CCGG-CTTGAGTGATAAAGAACCTCT-CTCGAG-AGAGGTTCTTTATCACTCAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGAGTGATAAAGAACCTCTCTCGAGAGAGGTTCTTTATCACTCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGAGTGATAAAGAACCTCTCTCGAGAGAGGTTCTTTATCACTCAAG-3'

Other clones with same target sequence:

(none)