Construct: shRNA TRCN0000221176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021462.2-367s1c2, TRCN0000024079, NM_021462.2-367s1c1
DNA Barcode:
GACCGTTTCTACCTGGTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mknk2 (17347)
Gene Description:
MAP kinase-interacting serine/threonine kinase 2
Transcript:
RefSeq NM_021462.2 (NON-CURRENT)
Match location:
Position 367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCGTTTCTACCTGGTGTTT
Hairpin Sequence:
5'-CCGG-GACCGTTTCTACCTGGTGTTT-CTCGAG-AAACACCAGGTAGAAACGGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCGTTTCTACCTGGTGTTTCTCGAGAAACACCAGGTAGAAACGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCGTTTCTACCTGGTGTTTCTCGAGAAACACCAGGTAGAAACGGTC-3'

Other clones with same target sequence:

  1. TRCN0000297864