Construct: shRNA TRCN0000221200

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022801.1-887s1c2, TRCN0000024104, NM_022801.1-887s1c1
DNA Barcode:
CGGGAAGTACAGAATCCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mark3 (17169)
Gene Description:
MAP/microtubule affinity regulating kinase 3
Transcript:
RefSeq NM_022801.1 (NON-CURRENT)
Match location:
Position 887 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221200)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAAGTACAGAATCCCTTT
Hairpin Sequence:
5'-CCGG-CGGGAAGTACAGAATCCCTTT-CTCGAG-AAAGGGATTCTGTACTTCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAAGTACAGAATCCCTTTCTCGAGAAAGGGATTCTGTACTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAAGTACAGAATCCCTTTCTCGAGAAAGGGATTCTGTACTTCCCG-3'

Other clones with same target sequence:

(none)