Construct: shRNA TRCN0000221208
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_196007.3-1659s1c2, TRCN0000024112, XM_196007.3-1659s1c1
- DNA Barcode:
- GCAGAGAGAATCTGGCTACTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nuak1 (77976)
- Gene Description:
- NUAK family, SNF1-like kinase, 1
- Transcript:
- RefSeq XM_196007.3 (NON-CURRENT)
- Match location:
- Position 1659 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000221208)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGAGAGAATCTGGCTACTA
- Hairpin Sequence:
-
5'-CCGG-GCAGAGAGAATCTGGCTACTA-CTCGAG-TAGTAGCCAGATTCTCTCTGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGAGAGAATCTGGCTACTACTCGAGTAGTAGCCAGATTCTCTCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGAGAGAATCTGGCTACTACTCGAGTAGTAGCCAGATTCTCTCTGC-3'
Other clones with same target sequence:
-
TRCN0000321730
