Construct: shRNA TRCN0000221208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_196007.3-1659s1c2, TRCN0000024112, XM_196007.3-1659s1c1
DNA Barcode:
GCAGAGAGAATCTGGCTACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nuak1 (77976)
Gene Description:
NUAK family, SNF1-like kinase, 1
Transcript:
RefSeq XM_196007.3 (NON-CURRENT)
Match location:
Position 1659 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAGAATCTGGCTACTA
Hairpin Sequence:
5'-CCGG-GCAGAGAGAATCTGGCTACTA-CTCGAG-TAGTAGCCAGATTCTCTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAGAATCTGGCTACTACTCGAGTAGTAGCCAGATTCTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAGAATCTGGCTACTACTCGAGTAGTAGCCAGATTCTCTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000321730