Construct: shRNA TRCN0000221229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358897.1-1192s1c2, TRCN0000024134, XM_358897.1-1192s1c1
DNA Barcode:
GCAGTGTGACATTTGGAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stk33 (117229)
Gene Description:
serine/threonine kinase 33
Transcript:
RefSeq XM_358897.1 (NON-CURRENT)
Match location:
Position 1192 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGTGACATTTGGAGCAT
Hairpin Sequence:
5'-CCGG-GCAGTGTGACATTTGGAGCAT-CTCGAG-ATGCTCCAAATGTCACACTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGTGACATTTGGAGCATCTCGAGATGCTCCAAATGTCACACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGTGACATTTGGAGCATCTCGAGATGCTCCAAATGTCACACTGC-3'

Other clones with same target sequence:

(none)