Construct: shRNA TRCN0000221253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019978.1-1528s1c2, TRCN0000024159, NM_019978.1-1528s1c1
DNA Barcode:
CCCAACATTGTCCTCCTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dclk1 (13175)
Gene Description:
doublecortin-like kinase 1
Transcript:
RefSeq NM_019978.1 (NON-CURRENT)
Match location:
Position 1528 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACATTGTCCTCCTGATT
Hairpin Sequence:
5'-CCGG-CCCAACATTGTCCTCCTGATT-CTCGAG-AATCAGGAGGACAATGTTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACATTGTCCTCCTGATTCTCGAGAATCAGGAGGACAATGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACATTGTCCTCCTGATTCTCGAGAATCAGGAGGACAATGTTGGG-3'

Other clones with same target sequence:

(none)