Construct: shRNA TRCN0000221268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183168.1-703s1c2, TRCN0000028358, NM_183168.1-703s1c1
DNA Barcode:
CGCTTCCTCTTCTATGCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
P2ry6 (233571)
Gene Description:
pyrimidinergic receptor P2Y, G-protein coupled, 6
Transcript:
RefSeq NM_183168.1 (NON-CURRENT)
Match location:
Position 703 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTCCTCTTCTATGCCAAT
Hairpin Sequence:
5'-CCGG-CGCTTCCTCTTCTATGCCAAT-CTCGAG-ATTGGCATAGAAGAGGAAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTCCTCTTCTATGCCAATCTCGAGATTGGCATAGAAGAGGAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTCCTCTTCTATGCCAATCTCGAGATTGGCATAGAAGAGGAAGCG-3'

Other clones with same target sequence:

(none)