Construct: shRNA TRCN0000221318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009953.1-2250s1c2, TRCN0000028326, NM_009953.1-2250s1c1
DNA Barcode:
CCACAATTCAAGCAACGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crhr2 (12922)
Gene Description:
corticotropin releasing hormone receptor 2
Transcript:
RefSeq NM_009953.1 (NON-CURRENT)
Match location:
Position 2250 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAATTCAAGCAACGAGAT
Hairpin Sequence:
5'-CCGG-CCACAATTCAAGCAACGAGAT-CTCGAG-ATCTCGTTGCTTGAATTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAATTCAAGCAACGAGATCTCGAGATCTCGTTGCTTGAATTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAATTCAAGCAACGAGATCTCGAGATCTCGTTGCTTGAATTGTGG-3'

Other clones with same target sequence:

(none)