Construct: shRNA TRCN0000221334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010098.1-1093s1c2, TRCN0000028325, NM_010098.1-1093s1c1
DNA Barcode:
CCCATCGTGATGTCACAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Opn3 (13603)
Gene Description:
opsin 3
Transcript:
RefSeq NM_010098.1 (NON-CURRENT)
Match location:
Position 1093 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCGTGATGTCACAGAAA
Hairpin Sequence:
5'-CCGG-CCCATCGTGATGTCACAGAAA-CTCGAG-TTTCTGTGACATCACGATGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCGTGATGTCACAGAAACTCGAGTTTCTGTGACATCACGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCGTGATGTCACAGAAACTCGAGTTTCTGTGACATCACGATGGG-3'

Other clones with same target sequence:

(none)