Construct: shRNA TRCN0000221344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002046.2-649s1c2, TRCN0000025836, NM_002046.2-649s1c1
DNA Barcode:
TCCGGGAAACTGTGGCGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAPDH (2597)
Gene Description:
glyceraldehyde-3-phosphate dehydrogenase
Transcript:
RefSeq NM_002046.2 (NON-CURRENT)
Match location:
Position 649 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGGGAAACTGTGGCGTGAT
Hairpin Sequence:
5'-CCGG-TCCGGGAAACTGTGGCGTGAT-CTCGAG-ATCACGCCACAGTTTCCCGGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGGGAAACTGTGGCGTGATCTCGAGATCACGCCACAGTTTCCCGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGGGAAACTGTGGCGTGATCTCGAGATCACGCCACAGTTTCCCGGA-3'

Other clones with same target sequence:

(none)