Construct: shRNA TRCN0000221384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000110.2-3009s1c2, TRCN0000025809, NM_000110.2-3009s1c1
DNA Barcode:
GCTATACAGTTTGATCCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPYD (1806)
Gene Description:
dihydropyrimidine dehydrogenase
Transcript:
RefSeq NM_000110.2 (NON-CURRENT)
Match location:
Position 3009 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATACAGTTTGATCCAGAA
Hairpin Sequence:
5'-CCGG-GCTATACAGTTTGATCCAGAA-CTCGAG-TTCTGGATCAAACTGTATAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATACAGTTTGATCCAGAACTCGAGTTCTGGATCAAACTGTATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATACAGTTTGATCCAGAACTCGAGTTCTGGATCAAACTGTATAGC-3'

Other clones with same target sequence:

(none)