Construct: shRNA TRCN0000221398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_007651.13-544s1c2, TRCN0000025787, XM_007651.13-544s1c1
DNA Barcode:
GCTCAAGTAGTGGTGACTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SORDL (116166)
Gene Description:
sorbitol dehydrogenase-like
Transcript:
RefSeq XM_007651.13 (NON-CURRENT)
Match location:
Position 544 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAAGTAGTGGTGACTGAT
Hairpin Sequence:
5'-CCGG-GCTCAAGTAGTGGTGACTGAT-CTCGAG-ATCAGTCACCACTACTTGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAAGTAGTGGTGACTGATCTCGAGATCAGTCACCACTACTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAAGTAGTGGTGACTGATCTCGAGATCAGTCACCACTACTTGAGC-3'

Other clones with same target sequence:

(none)