Construct: shRNA TRCN0000221426

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001918.1-954s1c2, TRCN0000025837, NM_001918.1-954s1c1
DNA Barcode:
GCTGCTTCCTTGGGATTACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DBT (1629)
Gene Description:
dihydrolipoamide branched chain transacylase E2
Transcript:
RefSeq NM_001918.1 (NON-CURRENT)
Match location:
Position 954 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221426)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTTCCTTGGGATTACTA
Hairpin Sequence:
5'-CCGG-GCTGCTTCCTTGGGATTACTA-CTCGAG-TAGTAATCCCAAGGAAGCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTTCCTTGGGATTACTACTCGAGTAGTAATCCCAAGGAAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTTCCTTGGGATTACTACTCGAGTAGTAATCCCAAGGAAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000319137