Construct: shRNA TRCN0000221451

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013628.1-2350s1c2, TRCN0000032919, NM_013628.1-2350s1c1
DNA Barcode:
GCCTGAGTTGGGAATCTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcsk1 (18548)
Gene Description:
proprotein convertase subtilisin/kexin type 1
Transcript:
RefSeq NM_013628.1 (NON-CURRENT)
Match location:
Position 2350 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221451)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAGTTGGGAATCTTCAT
Hairpin Sequence:
5'-CCGG-GCCTGAGTTGGGAATCTTCAT-CTCGAG-ATGAAGATTCCCAACTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAGTTGGGAATCTTCATCTCGAGATGAAGATTCCCAACTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAGTTGGGAATCTTCATCTCGAGATGAAGATTCCCAACTCAGGC-3'

Other clones with same target sequence:

(none)