Construct: shRNA TRCN0000221485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008792.3-1615s1c2, TRCN0000032954, NM_008792.3-1615s1c1
DNA Barcode:
CCTGGAATTTAATCACCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcsk2 (18549)
Gene Description:
proprotein convertase subtilisin/kexin type 2
Transcript:
RefSeq NM_008792.3 (NON-CURRENT)
Match location:
Position 1615 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAATTTAATCACCTCTT
Hairpin Sequence:
5'-CCGG-CCTGGAATTTAATCACCTCTT-CTCGAG-AAGAGGTGATTAAATTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAATTTAATCACCTCTTCTCGAGAAGAGGTGATTAAATTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAATTTAATCACCTCTTCTCGAGAAGAGGTGATTAAATTCCAGG-3'

Other clones with same target sequence:

(none)