Construct: shRNA TRCN0000221502
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011985.1-630s1c2, TRCN0000032971, NM_011985.1-630s1c1
- DNA Barcode:
- GATAGGTTTCTACCCAGTCAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mmp23 (26561)
- Gene Description:
- matrix metallopeptidase 23
- Transcript:
- RefSeq NM_011985.1 (NON-CURRENT)
- Match location:
- Position 630 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000221502)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GATAGGTTTCTACCCAGTCAA
- Hairpin Sequence:
-
5'-CCGG-GATAGGTTTCTACCCAGTCAA-CTCGAG-TTGACTGGGTAGAAACCTATC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGATAGGTTTCTACCCAGTCAACTCGAGTTGACTGGGTAGAAACCTATCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGATAGGTTTCTACCCAGTCAACTCGAGTTGACTGGGTAGAAACCTATC-3'
Other clones with same target sequence:
-
TRCN0000352265
