Construct: shRNA TRCN0000221517
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_002953.2-1406s1c2, TRCN0000039755, NM_002953.2-1406s1c1
- DNA Barcode:
- GCTACGTGGTAAAGGAGACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA1 (6195)
- Gene Description:
- ribosomal protein S6 kinase A1
- Transcript:
- RefSeq NM_002953.2 (NON-CURRENT)
- Match location:
- Position 1406 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000221517)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTACGTGGTAAAGGAGACAA
- Hairpin Sequence:
-
5'-CCGG-GCTACGTGGTAAAGGAGACAA-CTCGAG-TTGTCTCCTTTACCACGTAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTACGTGGTAAAGGAGACAACTCGAGTTGTCTCCTTTACCACGTAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTACGTGGTAAAGGAGACAACTCGAGTTGTCTCCTTTACCACGTAGC-3'
Other clones with same target sequence:
(none)
