Construct: shRNA TRCN0000221532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020761.1-795s1c2, TRCN0000039772, NM_020761.1-795s1c1
DNA Barcode:
CGAGTCCTCTTTCACTACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPTOR (57521)
Gene Description:
regulatory associated protein of MTOR complex 1
Transcript:
RefSeq NM_020761.1 (NON-CURRENT)
Match location:
Position 795 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTCCTCTTTCACTACAAT
Hairpin Sequence:
5'-CCGG-CGAGTCCTCTTTCACTACAAT-CTCGAG-ATTGTAGTGAAAGAGGACTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTCCTCTTTCACTACAATCTCGAGATTGTAGTGAAAGAGGACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTCCTCTTTCACTACAATCTCGAGATTGTAGTGAAAGAGGACTCG-3'

Other clones with same target sequence:

  1. TRCN0000332886