Construct: shRNA TRCN0000221544

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004958.2-5477s1c2, TRCN0000039785, NM_004958.2-5477s1c1
DNA Barcode:
GCTGTGCTACACTACAAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTOR (2475)
Gene Description:
mechanistic target of rapamycin kinase
Transcript:
RefSeq NM_004958.2 (NON-CURRENT)
Match location:
Position 5477 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221544)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGCTACACTACAAACAT
Hairpin Sequence:
5'-CCGG-GCTGTGCTACACTACAAACAT-CTCGAG-ATGTTTGTAGTGTAGCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGCTACACTACAAACATCTCGAGATGTTTGTAGTGTAGCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGCTACACTACAAACATCTCGAGATGTTTGTAGTGTAGCACAGC-3'

Other clones with same target sequence:

  1. TRCN0000332888