Construct: shRNA TRCN0000221554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005163.1-1410s1c2, TRCN0000039796, NM_005163.1-1410s1c1
DNA Barcode:
GCATCGCTTCTTTGCCGGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKT1 (207)
Gene Description:
AKT serine/threonine kinase 1
Transcript:
RefSeq NM_005163.1 (NON-CURRENT)
Match location:
Position 1410 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCGCTTCTTTGCCGGTAT
Hairpin Sequence:
5'-CCGG-GCATCGCTTCTTTGCCGGTAT-CTCGAG-ATACCGGCAAAGAAGCGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCGCTTCTTTGCCGGTATCTCGAGATACCGGCAAAGAAGCGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCGCTTCTTTGCCGGTATCTCGAGATACCGGCAAAGAAGCGATGC-3'

Other clones with same target sequence:

(none)