Construct: shRNA TRCN0000221571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005215.1-2265s1c2, TRCN0000039814, NM_005215.1-2265s1c1
DNA Barcode:
GCGAGGTTATATTATCGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCC (1630)
Gene Description:
DCC netrin 1 receptor
Transcript:
RefSeq NM_005215.1 (NON-CURRENT)
Match location:
Position 2265 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGGTTATATTATCGGTTA
Hairpin Sequence:
5'-CCGG-GCGAGGTTATATTATCGGTTA-CTCGAG-TAACCGATAATATAACCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGGTTATATTATCGGTTACTCGAGTAACCGATAATATAACCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGGTTATATTATCGGTTACTCGAGTAACCGATAATATAACCTCGC-3'

Other clones with same target sequence:

(none)