Construct: shRNA TRCN0000221578
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004960.1-1735s1c2, TRCN0000039823, NM_004960.1-1735s1c1
- DNA Barcode:
- TTCCTGATCACCCAAGGGTTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FUS (2521)
- Gene Description:
- FUS RNA binding protein
- Transcript:
- RefSeq NM_004960.1 (NON-CURRENT)
- Match location:
- Position 1735 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000221578)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TTCCTGATCACCCAAGGGTTC
- Hairpin Sequence:
-
5'-CCGG-TTCCTGATCACCCAAGGGTTC-CTCGAG-GAACCCTTGGGTGATCAGGAA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTTCCTGATCACCCAAGGGTTCCTCGAGGAACCCTTGGGTGATCAGGAATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATTCCTGATCACCCAAGGGTTCCTCGAGGAACCCTTGGGTGATCAGGAA-3'
Other clones with same target sequence:
(none)
