Construct: shRNA TRCN0000221598

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004592.2-2075s1c2, TRCN0000017225, NM_004592.2-2075s1c1
DNA Barcode:
GCTAGAAGCAAAGCAAGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SFSWAP (6433)
Gene Description:
splicing factor SWAP
Transcript:
RefSeq NM_004592.2 (NON-CURRENT)
Match location:
Position 2075 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221598)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGAAGCAAAGCAAGCAAA
Hairpin Sequence:
5'-CCGG-GCTAGAAGCAAAGCAAGCAAA-CTCGAG-TTTGCTTGCTTTGCTTCTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGAAGCAAAGCAAGCAAACTCGAGTTTGCTTGCTTTGCTTCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGAAGCAAAGCAAGCAAACTCGAGTTTGCTTGCTTTGCTTCTAGC-3'

Other clones with same target sequence:

  1. TRCN0000275299