Construct: shRNA TRCN0000221678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003410.1-963s1c2, TRCN0000017309, NM_003410.1-963s1c1
DNA Barcode:
GTCGGAAATTGATCCTTGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFX (7543)
Gene Description:
zinc finger protein X-linked
Transcript:
RefSeq NM_003410.1 (NON-CURRENT)
Match location:
Position 963 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGGAAATTGATCCTTGTAA
Hairpin Sequence:
5'-CCGG-GTCGGAAATTGATCCTTGTAA-CTCGAG-TTACAAGGATCAATTTCCGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGGAAATTGATCCTTGTAACTCGAGTTACAAGGATCAATTTCCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGGAAATTGATCCTTGTAACTCGAGTTACAAGGATCAATTTCCGAC-3'

Other clones with same target sequence:

(none)