Construct: shRNA TRCN0000221711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145419.1-2750s1c2, TRCN0000025453, NM_145419.1-2750s1c1
DNA Barcode:
GTGACATTTATGTTGTCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hkdc1 (216019)
Gene Description:
hexokinase domain containing 1
Transcript:
RefSeq NM_145419.1 (CURRENT)
Match location:
Position 2750 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGACATTTATGTTGTCAGAA
Hairpin Sequence:
5'-CCGG-GTGACATTTATGTTGTCAGAA-CTCGAG-TTCTGACAACATAAATGTCAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGACATTTATGTTGTCAGAACTCGAGTTCTGACAACATAAATGTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGACATTTATGTTGTCAGAACTCGAGTTCTGACAACATAAATGTCAC-3'

Other clones with same target sequence:

(none)