Construct: shRNA TRCN0000221728

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011158.2-741s1c2, TRCN0000025470, NM_011158.2-741s1c1
DNA Barcode:
CGTCATTGACAGAGGAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkar2b (19088)
Gene Description:
protein kinase, cAMP dependent regulatory, type II beta
Transcript:
RefSeq NM_011158.2 (NON-CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221728)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCATTGACAGAGGAACATT
Hairpin Sequence:
5'-CCGG-CGTCATTGACAGAGGAACATT-CTCGAG-AATGTTCCTCTGTCAATGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCATTGACAGAGGAACATTCTCGAGAATGTTCCTCTGTCAATGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCATTGACAGAGGAACATTCTCGAGAATGTTCCTCTGTCAATGACG-3'

Other clones with same target sequence:

(none)