Construct: shRNA TRCN0000221742
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021880.1-1161s1c2, TRCN0000025484, NM_021880.1-1161s1c1
- DNA Barcode:
- CCCTTTGAAGTGCGTTAAGTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Prkar1a (19084)
- Gene Description:
- protein kinase, cAMP dependent regulatory, type I, alpha
- Transcript:
- RefSeq NM_021880.1 (NON-CURRENT)
- Match location:
- Position 1161 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000221742)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCTTTGAAGTGCGTTAAGTT
- Hairpin Sequence:
-
5'-CCGG-CCCTTTGAAGTGCGTTAAGTT-CTCGAG-AACTTAACGCACTTCAAAGGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCTTTGAAGTGCGTTAAGTTCTCGAGAACTTAACGCACTTCAAAGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCTTTGAAGTGCGTTAAGTTCTCGAGAACTTAACGCACTTCAAAGGG-3'
Other clones with same target sequence:
-
TRCN0000274643
