Construct: shRNA TRCN0000221763

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_133060.2-553s1c2, TRCN0000025505, XM_133060.2-553s1c1
DNA Barcode:
GCTGACTACCTTCTTCAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrguk (74354)
Gene Description:
leucine-rich repeats and guanylate kinase domain containing
Transcript:
RefSeq XM_133060.2 (NON-CURRENT)
Match location:
Position 553 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221763)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTACCTTCTTCAATTT
Hairpin Sequence:
5'-CCGG-GCTGACTACCTTCTTCAATTT-CTCGAG-AAATTGAAGAAGGTAGTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTACCTTCTTCAATTTCTCGAGAAATTGAAGAAGGTAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTACCTTCTTCAATTTCTCGAGAAATTGAAGAAGGTAGTCAGC-3'

Other clones with same target sequence:

(none)