Construct: shRNA TRCN0000221806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018782.1-233s1c2, TRCN0000026665, NM_018782.1-233s1c1
DNA Barcode:
GCCTCTTAATATGGCTCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Calcrl (54598)
Gene Description:
calcitonin receptor-like
Transcript:
RefSeq NM_018782.1 (NON-CURRENT)
Match location:
Position 233 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTAATATGGCTCTCAT
Hairpin Sequence:
5'-CCGG-GCCTCTTAATATGGCTCTCAT-CTCGAG-ATGAGAGCCATATTAAGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTAATATGGCTCTCATCTCGAGATGAGAGCCATATTAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTAATATGGCTCTCATCTCGAGATGAGAGCCATATTAAGAGGC-3'

Other clones with same target sequence:

(none)