Construct: shRNA TRCN0000221855

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178712.2-1926s1c2, TRCN0000026635, NM_178712.2-1926s1c1
DNA Barcode:
GCTCTGACATTTATCACGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrg2 (237175)
Gene Description:
adhesion G protein-coupled receptor G2
Transcript:
RefSeq NM_178712.2 (NON-CURRENT)
Match location:
Position 1926 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221855)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGACATTTATCACGTAT
Hairpin Sequence:
5'-CCGG-GCTCTGACATTTATCACGTAT-CTCGAG-ATACGTGATAAATGTCAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGACATTTATCACGTATCTCGAGATACGTGATAAATGTCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGACATTTATCACGTATCTCGAGATACGTGATAAATGTCAGAGC-3'

Other clones with same target sequence:

(none)