Construct: shRNA TRCN0000221872
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_000668.3-182s1c2, TRCN0000028549, NM_000668.3-182s1c1
- DNA Barcode:
- CGCATTAAGATGGTGGCTGTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADH1B (125)
- Gene Description:
- alcohol dehydrogenase 1B (class I), beta polypeptide
- Transcript:
- RefSeq NM_000668.3 (NON-CURRENT)
- Match location:
- Position 182 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000221872)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGCATTAAGATGGTGGCTGTA
- Hairpin Sequence:
-
5'-CCGG-CGCATTAAGATGGTGGCTGTA-CTCGAG-TACAGCCACCATCTTAATGCG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGCATTAAGATGGTGGCTGTACTCGAGTACAGCCACCATCTTAATGCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGCATTAAGATGGTGGCTGTACTCGAGTACAGCCACCATCTTAATGCG-3'
Other clones with same target sequence:
(none)
