Construct: shRNA TRCN0000221879

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182977.1-181s1c2, TRCN0000028489, NM_182977.1-181s1c1
DNA Barcode:
CGTGTCCTCTACTTAGCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NNT (23530)
Gene Description:
nicotinamide nucleotide transhydrogenase
Transcript:
RefSeq NM_182977.1 (NON-CURRENT)
Match location:
Position 181 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221879)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTCCTCTACTTAGCAATT
Hairpin Sequence:
5'-CCGG-CGTGTCCTCTACTTAGCAATT-CTCGAG-AATTGCTAAGTAGAGGACACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTCCTCTACTTAGCAATTCTCGAGAATTGCTAAGTAGAGGACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTCCTCTACTTAGCAATTCTCGAGAATTGCTAAGTAGAGGACACG-3'

Other clones with same target sequence:

  1. TRCN0000278311