Construct: shRNA TRCN0000221884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005918.2-417s1c2, TRCN0000028561, NM_005918.2-417s1c1
DNA Barcode:
GACGACCTGTTCAACACCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MDH2 (4191)
Gene Description:
malate dehydrogenase 2
Transcript:
RefSeq NM_005918.2 (NON-CURRENT)
Match location:
Position 417 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGACCTGTTCAACACCAAT
Hairpin Sequence:
5'-CCGG-GACGACCTGTTCAACACCAAT-CTCGAG-ATTGGTGTTGAACAGGTCGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGACCTGTTCAACACCAATCTCGAGATTGGTGTTGAACAGGTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGACCTGTTCAACACCAATCTCGAGATTGGTGTTGAACAGGTCGTC-3'

Other clones with same target sequence:

  1. TRCN0000278262