Construct: shRNA TRCN0000221887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000667.2-1176s1c2, TRCN0000028516, NM_000667.2-1176s1c1
DNA Barcode:
ATCCGTACCATTCTGATGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADH1A (124)
Gene Description:
alcohol dehydrogenase 1A (class I), alpha polypeptide
Transcript:
RefSeq NM_000667.2 (NON-CURRENT)
Match location:
Position 1176 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCGTACCATTCTGATGTTT
Hairpin Sequence:
5'-CCGG-ATCCGTACCATTCTGATGTTT-CTCGAG-AAACATCAGAATGGTACGGAT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCGTACCATTCTGATGTTTCTCGAGAAACATCAGAATGGTACGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCGTACCATTCTGATGTTTCTCGAGAAACATCAGAATGGTACGGAT-3'

Other clones with same target sequence:

(none)