Construct: shRNA TRCN0000221901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012212.2-720s1c2, TRCN0000028550, NM_012212.2-720s1c1
DNA Barcode:
CGTCTCCTGATGGTTATGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTGR1 (22949)
Gene Description:
prostaglandin reductase 1
Transcript:
RefSeq NM_012212.2 (NON-CURRENT)
Match location:
Position 720 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTCCTGATGGTTATGATT
Hairpin Sequence:
5'-CCGG-CGTCTCCTGATGGTTATGATT-CTCGAG-AATCATAACCATCAGGAGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTCCTGATGGTTATGATTCTCGAGAATCATAACCATCAGGAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTCCTGATGGTTATGATTCTCGAGAATCATAACCATCAGGAGACG-3'

Other clones with same target sequence:

  1. TRCN0000343529