Construct: shRNA TRCN0000221912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152740.2-593s1c2, TRCN0000028504, NM_152740.2-593s1c1
DNA Barcode:
GCACTATTGATCCTGCAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HIBADH (11112)
Gene Description:
3-hydroxyisobutyrate dehydrogenase
Transcript:
RefSeq NM_152740.2 (NON-CURRENT)
Match location:
Position 593 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTATTGATCCTGCAGTTT
Hairpin Sequence:
5'-CCGG-GCACTATTGATCCTGCAGTTT-CTCGAG-AAACTGCAGGATCAATAGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTATTGATCCTGCAGTTTCTCGAGAAACTGCAGGATCAATAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTATTGATCCTGCAGTTTCTCGAGAAACTGCAGGATCAATAGTGC-3'

Other clones with same target sequence:

(none)