Construct: shRNA TRCN0000221915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152740.2-1004s1c2, TRCN0000028495, NM_152740.2-1004s1c1
DNA Barcode:
CTAATAACTATCAGGGTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HIBADH (11112)
Gene Description:
3-hydroxyisobutyrate dehydrogenase
Transcript:
RefSeq NM_152740.2 (NON-CURRENT)
Match location:
Position 1004 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAATAACTATCAGGGTGGAT
Hairpin Sequence:
5'-CCGG-CTAATAACTATCAGGGTGGAT-CTCGAG-ATCCACCCTGATAGTTATTAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAATAACTATCAGGGTGGATCTCGAGATCCACCCTGATAGTTATTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAATAACTATCAGGGTGGATCTCGAGATCCACCCTGATAGTTATTAG-3'

Other clones with same target sequence:

(none)