Construct: shRNA TRCN0000221941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001608.2-1384s1c2, TRCN0000028528, NM_001608.2-1384s1c1
DNA Barcode:
GCCAATCTATGGTGGTACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACADL (33)
Gene Description:
acyl-CoA dehydrogenase long chain
Transcript:
RefSeq NM_001608.2 (NON-CURRENT)
Match location:
Position 1384 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATCTATGGTGGTACAAA
Hairpin Sequence:
5'-CCGG-GCCAATCTATGGTGGTACAAA-CTCGAG-TTTGTACCACCATAGATTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATCTATGGTGGTACAAACTCGAGTTTGTACCACCATAGATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATCTATGGTGGTACAAACTCGAGTTTGTACCACCATAGATTGGC-3'

Other clones with same target sequence:

(none)