Construct: shRNA TRCN0000221973

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144537.1-1365s1c2, TRCN0000033014, NM_144537.1-1365s1c1
DNA Barcode:
CGGAGGAAATGGTTCTAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpa5 (74649)
Gene Description:
carboxypeptidase A5
Transcript:
RefSeq NM_144537.1 (NON-CURRENT)
Match location:
Position 1365 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221973)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGGAAATGGTTCTAACAA
Hairpin Sequence:
5'-CCGG-CGGAGGAAATGGTTCTAACAA-CTCGAG-TTGTTAGAACCATTTCCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGGAAATGGTTCTAACAACTCGAGTTGTTAGAACCATTTCCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGGAAATGGTTCTAACAACTCGAGTTGTTAGAACCATTTCCTCCG-3'

Other clones with same target sequence:

(none)