Construct: shRNA TRCN0000221981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355911.1-1209s1c2, TRCN0000033022, XM_355911.1-1209s1c1
DNA Barcode:
GTGTCGAAGATGTGAGGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcsk6 (18553)
Gene Description:
proprotein convertase subtilisin/kexin type 6
Transcript:
RefSeq XM_355911.1 (NON-CURRENT)
Match location:
Position 1209 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTCGAAGATGTGAGGAGAA
Hairpin Sequence:
5'-CCGG-GTGTCGAAGATGTGAGGAGAA-CTCGAG-TTCTCCTCACATCTTCGACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTCGAAGATGTGAGGAGAACTCGAGTTCTCCTCACATCTTCGACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTCGAAGATGTGAGGAGAACTCGAGTTCTCCTCACATCTTCGACAC-3'

Other clones with same target sequence:

  1. TRCN0000334330