Construct: shRNA TRCN0000221989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053122.2-470s1c2, TRCN0000033030, NM_053122.2-470s1c1
DNA Barcode:
GCGATCATCATGGACACAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Immp2l (93757)
Gene Description:
IMP2 inner mitochondrial membrane peptidase-like (S. cerevisiae)
Transcript:
RefSeq NM_053122.2 (NON-CURRENT)
Match location:
Position 470 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATCATCATGGACACAGTT
Hairpin Sequence:
5'-CCGG-GCGATCATCATGGACACAGTT-CTCGAG-AACTGTGTCCATGATGATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATCATCATGGACACAGTTCTCGAGAACTGTGTCCATGATGATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATCATCATGGACACAGTTCTCGAGAACTGTGTCCATGATGATCGC-3'

Other clones with same target sequence:

(none)