Construct: shRNA TRCN0000222010

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030703.1-1087s1c2, TRCN0000033053, NM_030703.1-1087s1c1
DNA Barcode:
GTATTCTCTCAGCAAGGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpn1 (93721)
Gene Description:
carboxypeptidase N, polypeptide 1
Transcript:
RefSeq NM_030703.1 (NON-CURRENT)
Match location:
Position 1087 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222010)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATTCTCTCAGCAAGGGAAT
Hairpin Sequence:
5'-CCGG-GTATTCTCTCAGCAAGGGAAT-CTCGAG-ATTCCCTTGCTGAGAGAATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATTCTCTCAGCAAGGGAATCTCGAGATTCCCTTGCTGAGAGAATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATTCTCTCAGCAAGGGAATCTCGAGATTCCCTTGCTGAGAGAATAC-3'

Other clones with same target sequence:

  1. TRCN0000312340