Construct: shRNA TRCN0000222012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_129214.2-2868s1c2, TRCN0000033055, XM_129214.2-2868s1c1
DNA Barcode:
CCGGAGCAGATGGATGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcsk5 (18552)
Gene Description:
proprotein convertase subtilisin/kexin type 5
Transcript:
RefSeq XM_129214.2 (NON-CURRENT)
Match location:
Position 2868 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGCAGATGGATGTATTA
Hairpin Sequence:
5'-CCGG-CCGGAGCAGATGGATGTATTA-CTCGAG-TAATACATCCATCTGCTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGCAGATGGATGTATTACTCGAGTAATACATCCATCTGCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGCAGATGGATGTATTACTCGAGTAATACATCCATCTGCTCCGG-3'

Other clones with same target sequence:

(none)