Construct: shRNA TRCN0000222019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029682.2-234s1c2, TRCN0000033063, NM_029682.2-234s1c1
DNA Barcode:
CCTAAGCAAACTTGGTTGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stambpl1 (76630)
Gene Description:
STAM binding protein like 1
Transcript:
RefSeq NM_029682.2 (NON-CURRENT)
Match location:
Position 234 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGCAAACTTGGTTGTAA
Hairpin Sequence:
5'-CCGG-CCTAAGCAAACTTGGTTGTAA-CTCGAG-TTACAACCAAGTTTGCTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGCAAACTTGGTTGTAACTCGAGTTACAACCAAGTTTGCTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGCAAACTTGGTTGTAACTCGAGTTACAACCAAGTTTGCTTAGG-3'

Other clones with same target sequence:

(none)