Construct: shRNA TRCN0000222023

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028260.1-296s1c2, TRCN0000033067, NM_028260.1-296s1c1
DNA Barcode:
CCATCAATGGAACCCACAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Immp1l (66541)
Gene Description:
IMP1 inner mitochondrial membrane peptidase-like (S. cerevisiae)
Transcript:
RefSeq NM_028260.1 (NON-CURRENT)
Match location:
Position 296 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222023)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAATGGAACCCACAATT
Hairpin Sequence:
5'-CCGG-CCATCAATGGAACCCACAATT-CTCGAG-AATTGTGGGTTCCATTGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAATGGAACCCACAATTCTCGAGAATTGTGGGTTCCATTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAATGGAACCCACAATTCTCGAGAATTGTGGGTTCCATTGATGG-3'

Other clones with same target sequence:

  1. TRCN0000302113