Construct: shRNA TRCN0000222048

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006479.2-420s1c2, TRCN0000062837, NM_006479.2-420s1c1
DNA Barcode:
CATATCTCTAATTGCAGTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAD51AP1 (10635)
Gene Description:
RAD51 associated protein 1
Transcript:
RefSeq NM_006479.2 (NON-CURRENT)
Match location:
Position 420 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222048)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATATCTCTAATTGCAGTGTA
Hairpin Sequence:
5'-CCGG-CATATCTCTAATTGCAGTGTA-CTCGAG-TACACTGCAATTAGAGATATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATATCTCTAATTGCAGTGTACTCGAGTACACTGCAATTAGAGATATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATATCTCTAATTGCAGTGTACTCGAGTACACTGCAATTAGAGATATG-3'

Other clones with same target sequence:

(none)