Construct: shRNA TRCN0000222053

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052884.1-1807s1c2, TRCN0000062842, NM_052884.1-1807s1c1
DNA Barcode:
GCTCGCTTTCTGTTCCTGCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIGLEC11 (114132)
Gene Description:
sialic acid binding Ig like lectin 11
Transcript:
RefSeq NM_052884.1 (NON-CURRENT)
Match location:
Position 1807 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222053)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGCTTTCTGTTCCTGCCT
Hairpin Sequence:
5'-CCGG-GCTCGCTTTCTGTTCCTGCCT-CTCGAG-AGGCAGGAACAGAAAGCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGCTTTCTGTTCCTGCCTCTCGAGAGGCAGGAACAGAAAGCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGCTTTCTGTTCCTGCCTCTCGAGAGGCAGGAACAGAAAGCGAGC-3'

Other clones with same target sequence:

(none)