Construct: shRNA TRCN0000222080

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003262.2-649s1c2, TRCN0000062871, NM_003262.2-649s1c1
DNA Barcode:
GAAGTTGGTGAACCATCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC62 (7095)
Gene Description:
SEC62 homolog, preprotein translocation factor
Transcript:
RefSeq NM_003262.2 (NON-CURRENT)
Match location:
Position 649 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222080)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTTGGTGAACCATCTAAA
Hairpin Sequence:
5'-CCGG-GAAGTTGGTGAACCATCTAAA-CTCGAG-TTTAGATGGTTCACCAACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTTGGTGAACCATCTAAACTCGAGTTTAGATGGTTCACCAACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTTGGTGAACCATCTAAACTCGAGTTTAGATGGTTCACCAACTTC-3'

Other clones with same target sequence:

  1. TRCN0000306854